4ry9

X-ray diffraction
1.4Å resolution

Crystal structure of carbohydrate transporter solute binding protein VEIS_2079 from Verminephrobacter eiseniae EF01-2, TARGET EFI-511009, a complex with D-TALITOL

Released:
Entry authors: Patskovsky Y, Toro R, Bhosle R, Al Obaidi N, Morisco LL, Wasserman SR, Chamala S, Attonito JD, Scott Glenn A, Chowdhury S, Lafleur J, Hillerich B, Siedel RD, Love J, Whalen KL, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peripla_BP_4 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 302 amino acids
Theoretical weight: 31.91 KDa
Source organism: Verminephrobacter eiseniae EF01-2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A1WJM2 (Residues: 32-330; Coverage: 100%)
Gene name: Veis_2079
Sequence domains: Periplasmic binding protein domain
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P2221
Unit cell:
a: 68.65Å b: 68.635Å c: 120.376Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.124 0.123 0.151
Expression system: Escherichia coli BL21(DE3)