X-ray diffraction
2.44Å resolution

Crystal structure of the CLR:RAMP1 extracellular domain heterodimer with bound high affinity CGRP analog


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Calcitonin gene-related peptide type 1 receptor; Maltose/maltodextrin-binding periplasmic protein; Receptor activity-modifying protein 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 593 amino acids
Theoretical weight: 66.3 KDa
Source organisms: Expression system: Escherichia coli
  • Canonical: P0AEX9 (Residues: 25-392; Coverage: 99%)
  • Canonical: O60894 (Residues: 23-108; Coverage: 67%)
  • Canonical: Q16602 (Residues: 31-144; Coverage: 26%)
Gene names: CALCRL, CGRPR, JW3994, RAMP1, b4034, malE
Sequence domains:
CGRP analog Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 12 amino acids
Theoretical weight: 1.19 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: C2
Unit cell:
a: 172.806Å b: 104.623Å c: 136.483Å
α: 90° β: 122.43° γ: 90°
R R work R free
0.202 0.2 0.243
Expression systems:
  • Escherichia coli
  • Not provided