X-ray diffraction
2.65Å resolution

2.65 Angstrom Resolution Crystal Structure of an orotate phosphoribosyltransferase from Bacillus anthracis str. 'Ames Ancestor' in complex with 5-phospho-alpha-D-ribosyl diphosphate (PRPP)

Source organism: Bacillus anthracis
Entry authors: Halavaty AS, Minasov G, Shuvalova L, Winsor J, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed:
Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Orotate phosphoribosyltransferase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 234 amino acids
Theoretical weight: 25.48 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q81WF6 (Residues: 1-210; Coverage: 100%)
Gene names: BAS3733, BA_4021, GBAA_4021, pyrE
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: I422
Unit cell:
a: 133.268Å b: 133.268Å c: 151.348Å
α: 90° β: 90° γ: 90°
R R work R free
0.198 0.196 0.242
Expression system: Escherichia coli BL21(DE3)