› 4ruu

X-ray diffraction
1.4Å resolution

Crystal structure of the Q108K:K40L mutant of human Cellular Retinol Binding ProteinII in complex with All-trans-Retinal after 24 hour incubation at 1.4 Angstrom Resolution

Released:
DOI: 10.2210/pdb4ruu/pdb
PDB 4ruu coloured by chain and viewed from the front.
PDB 4ruu coloured by chain and viewed from the side.
PDB 4ruu coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Tuning the electronic absorption of protein-embedded all-trans-retinal.
Wang W, Nossoni Z, Berbasova T, Watson CT, Yapici I, Lee KS, Vasileiou C, Geiger JH, Borhan B
Science 338 1340-3 (2012)
PMID: 23224553

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Retinol-binding protein 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 133 amino acids
Theoretical weight: 15.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P50120 (Residues: 2-134; Coverage: 99%)
Gene names: CRBP2, RBP2
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

2 bound ligands:
Ligand RET 2 x RET
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P1
Unit cell:
a: 29.479Å b: 36.57Å c: 64.093Å
α: 86.54° β: 86.6° γ: 64.83°
R-values:
R R work R free
0.209 0.207 0.238
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Towards translational optogenetics.
Bansal et al. (2022)
12 more