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Complex of Escherichia coli DNA Adenine Methyltransferase (DAM) with AdoMet and with DNA Containing Distal Pap Regulon Sequence

X-ray diffraction
2.5Å resolution
PDB entry 4rtm coloured by chain, front view.
PDB entry 4rtm coloured by chain, side view.
PDB entry 4rtm coloured by chain, top view.
Source organisms:
Assembly composition:
hetero trimer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: DNA adenine methylase and DNA
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Multimeric state: hetero trimer
Accessible surface area: 15435.33 Å2
Buried surface area: 1560.59 Å2
Dissociation area: 330.72 Å2
Dissociation energy (ΔGdiss): -2.61 kcal/mol
Dissociation entropy (TΔSdiss): 10.65 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-118143

Macromolecules

DNA adenine methylase
Chain: A
Length: 298 amino acids
Theoretical weight: 34.33 KDa
Source organism: Escherichia coli str. K-12 substr. MDS42
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEE8 (Residues: 1-278; Coverage: 100%)
Gene names: JW3350, b3387, dam
Pfam: D12 class N6 adenine-specific DNA methyltransferase
InterPro:
CATH:
DNA adenine methylase in PDB entry 4rtm, assembly 1, front view.
DNA adenine methylase in PDB entry 4rtm, assembly 1, side view.
DNA adenine methylase in PDB entry 4rtm, assembly 1, top view.
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DNA chain F
Name: DNA (5'-D(*TP*CP*TP*AP*AP*AP*GP*AP*TP*CP*G)-3')
Representative chains: F
Source organism: synthetic construct [32630]
Expression system: Not provided
Length: 11 nucleotides
Theoretical weight: 3.36 KDa
DNA (5'-D(*TP*CP*TP*AP*AP*AP*GP*AP*TP*CP*G)-3') in PDB entry 4rtm, assembly 1, front view.
DNA (5'-D(*TP*CP*TP*AP*AP*AP*GP*AP*TP*CP*G)-3') in PDB entry 4rtm, assembly 1, side view.
DNA (5'-D(*TP*CP*TP*AP*AP*AP*GP*AP*TP*CP*G)-3') in PDB entry 4rtm, assembly 1, top view.
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DNA chain G
Name: DNA (5'-D(*AP*CP*GP*AP*TP*CP*TP*TP*TP*AP*G)-3')
Representative chains: G
Source organism: synthetic construct [32630]
Expression system: Not provided
Length: 11 nucleotides
Theoretical weight: 3.35 KDa
DNA (5'-D(*AP*CP*GP*AP*TP*CP*TP*TP*TP*AP*G)-3') in PDB entry 4rtm, assembly 1, front view.
DNA (5'-D(*AP*CP*GP*AP*TP*CP*TP*TP*TP*AP*G)-3') in PDB entry 4rtm, assembly 1, side view.
DNA (5'-D(*AP*CP*GP*AP*TP*CP*TP*TP*TP*AP*G)-3') in PDB entry 4rtm, assembly 1, top view.
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