› 4rtj

X-ray diffraction
1.99Å resolution

A non-cognate complex of Escherichia coli DNA Adenine Methyltransferase (DAM) with DNA and Sinefungin

Released:
DOI: 10.2210/pdb4rtj/pdb
PDB 4rtj coloured by chain and viewed from the front.
PDB 4rtj coloured by chain and viewed from the side.
PDB 4rtj coloured by chain and viewed from the top.
Source organisms:
Primary publication:
Structures of Escherichia coli DNA adenine methyltransferase (Dam) in complex with a non-GATC sequence: potential implications for methylation-independent transcriptional repression.
Horton JR, Zhang X, Blumenthal RM, Cheng X
Nucleic Acids Res. (2015)
PMID: 25845600

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA adenine methylase Chain: A
Molecule details ›
Chain: A
Length: 298 amino acids
Theoretical weight: 34.33 KDa
Source organism: Escherichia coli str. K-12 substr. MDS42
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEE8 (Residues: 1-278; Coverage: 100%)
Gene names: JW3350, b3387, dam
Sequence domains: D12 class N6 adenine-specific DNA methyltransferase
Structure domains:
DNA (5'-D(*TP*CP*TP*AP*GP*AP*TP*CP*TP*AP*GP*A)-3') Chains: F, G
Molecule details ›
Chains: F, G
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments


Cofactor: Ligand SFG 1 x SFG
1 bound ligand:
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 36.367Å b: 63.114Å c: 145.043Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.214 0.236
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

6 citation in other articles

A role for the bacterial GATC methylome in antibiotic stress survival.
Cohen et al. (2016)
5 more