4rrp

X-ray diffraction
2.79Å resolution

Crystal Structure of the Fab complexed with antigen Asf1p, Northeast Structural Genomics Consortium (NESG) Target PdR16

Released:
Entry authors: Kuzin A, Lew S, Seetharaman J, Mao L, Xiao R, Oconnell PT, Maglaqui M, Bailey L, Everett JK, Acton TB, Montelione GT, Hunt JF, Tong L, Chaperone-Enabled Studies of Epigenetic Regulation Enzymes (CEBS), Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab antibody, light chain Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 216 amino acids
Theoretical weight: 23.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fab antibody, heavy chain Chains: G, H, I, J, K, L
Molecule details ›
Chains: G, H, I, J, K, L
Length: 238 amino acids
Theoretical weight: 25.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Antigen Asf1p Chains: M, N, O, P, Q, R
Molecule details ›
Chains: M, N, O, P, Q, R
Length: 160 amino acids
Theoretical weight: 18.06 KDa
Source organism: Saccharomyces cerevisiae AWRI796
Expression system: Escherichia coli
Structure domains: Histone chaperone ASF1-like

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X3A
Spacegroup: P212121
Unit cell:
a: 92.725Å b: 181.309Å c: 259.286Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.253
Expression system: Escherichia coli