PDBe 4rrf

X-ray diffraction
1.7Å resolution

Editing domain of threonyl-tRNA synthetase from Methanococcus jannaschii with L-Ser3AA

Released:

Function and Biology Details

Reaction catalysed:
ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Threonine--tRNA ligase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 141 amino acids
Theoretical weight: 15.95 KDa
Source organism: Methanocaldococcus jannaschii DSM 2661
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58597 (Residues: 1-141; Coverage: 23%)
Gene names: MJ1197, thrS
Sequence domains: Archaea-specific editing domain of threonyl-tRNA synthetase
Structure domains: D-tyrosyl-trna(Tyr) Deacylase; Chain: A;

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: C2
Unit cell:
a: 159.595Å b: 52.813Å c: 98.315Å
α: 90° β: 104.04° γ: 90°
R-values:
R R work R free
0.197 0.194 0.239
Expression system: Escherichia coli