› 4rqo

X-ray diffraction
2.25Å resolution

Crystal structure of L-Serine Dehydratase from Legionella pneumophila

Released:
DOI: 10.2210/pdb4rqo/pdb
PDB 4rqo coloured by chain and viewed from the front.
PDB 4rqo coloured by chain and viewed from the side.
PDB 4rqo coloured by chain and viewed from the top.
Source organism: Legionella pneumophila subsp. pneumophila str. Thunder Bay
Primary publication:
Structure of L-Serine Dehydratase from Legionella pneumophila: Novel Use of the C-terminal Cysteine as an Intrinsic Competitive Inhibitor.
Thoden JB, Holden HM, Grant GA
Biochemistry (2014)
PMID: 25380533

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-serine dehydratase Chains: A, B
Molecule details ›
Chains: A, B
Length: 476 amino acids
Theoretical weight: 52.6 KDa
Source organism: Legionella pneumophila subsp. pneumophila str. Thunder Bay
Expression system: Escherichia coli BL21(DE3)
Structure domains: D-3-phosphoglycerate dehydrogenase; domain 3

Ligands and Environments

2 bound ligands:
Ligand SF4 2 x SF4
Ligand CL 2 x CL
1 modified residue:
Ligand MSE 40 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P3121
Unit cell:
a: 81.39Å b: 81.39Å c: 267.521Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.198 0.258
Expression system: Escherichia coli BL21(DE3)

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Citations

3 citation in other articles

L-cysteine functionalized magnetic nanoparticles (LCMNP): a novel magnetically separable organocatalyst for one-pot synthesis of 2-amino-4H-chromene-3-carbonitriles in water.
Khalafi-Nezhad et al. (2015)
2 more

3 mentions without citation

Hydrogen-Deuterium Exchange Mass Spectrometry Reveals Unique Conformational and Chemical Transformations Occurring upon [4Fe-4S] Cluster Binding in the Type 2 L-Serine Dehydratase from Legionella pneumophila.
Yan et al. (2015)
2 more