› 4rpm

X-ray diffraction
1.4Å resolution

Crystal structure of the SAT domain from the non-reducing fungal polyketide synthase CazM with bound hexanoyl

Released:
DOI: 10.2210/pdb4rpm/pdb
PDB 4rpm coloured by chain and viewed from the front.
PDB 4rpm coloured by chain and viewed from the side.
PDB 4rpm coloured by chain and viewed from the top.
Source organism: Chaetomium globosum CBS 148.51
Entry authors: Winter JM, Cascio D, Sawaya MR, Tang Y

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SAT domain from CazM Chain: A
Molecule details ›
Chain: A
Length: 406 amino acids
Theoretical weight: 44.58 KDa
Source organism: Chaetomium globosum CBS 148.51
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2GWK9 (Residues: 2-113, 114-125, 126-378; Coverage: 32%)
Gene name: CHGG_07645
Sequence domains: Starter unit:ACP transacylase in aflatoxin biosynthesis

Ligands and Environments

2 bound ligands:
Ligand 6NA 1 x 6NA
Ligand HXC 1 x HXC
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 45.67Å b: 52.36Å c: 168.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.204
Expression system: Escherichia coli BL21(DE3)

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Citations

9 review citations

Chaetomugilins and Chaetoviridins-Promising Natural Metabolites: Structures, Separation, Characterization, Biosynthesis, Bioactivities, Molecular Docking, and Molecular Dynamics.
Omar et al. (2022)
8 more