› 4rpf

X-ray diffraction
2.3Å resolution

Crystal structure of homoserine kinase from Yersinia pestis Nepal516, NYSGRC target 032715

Released:
DOI: 10.2210/pdb4rpf/pdb
PDB 4rpf coloured by chain and viewed from the front.
PDB 4rpf coloured by chain and viewed from the side.
PDB 4rpf coloured by chain and viewed from the top.
Source organism: Yersinia pestis Nepal516
Entry authors: Ptskovsky Y, Bhosle R, Toro R, Hillerich B, Gizzi A, Garforth S, Kar A, Chan MK, Lafluer J, Patel H, Matikainen B, Chamala S, Lim S, Celikgil A, Villegas G, Evans B, Love J, Fiser A, Seidel R, Bonanno JB, Almo SC, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed: 
ATP + L-homoserine = ADP + O-phospho-L-homoserine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Homoserine kinase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 310 amino acids
Theoretical weight: 33.35 KDa
Source organism: Yersinia pestis Nepal516
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q1CMW6 (Residues: 1-309; Coverage: 100%)
Gene names: YP516_0338, YPN_0332, thrB
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CIT 3 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 162.861Å b: 93.659Å c: 107.617Å
α: 90° β: 90.14° γ: 90°
R-values:
R R work R free
0.242 0.24 0.291
Expression system: Escherichia coli BL21(DE3)

Quick links