4rpa

X-ray diffraction
2.1Å resolution

Crystal structure of inorganic pyrophosphatase from Staphylococcus aureus in complex with Mn2+

Released:

Function and Biology Details

Reaction catalysed:
Diphosphate + H(2)O = 2 phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable manganese-dependent inorganic pyrophosphatase Chains: A, B
Molecule details ›
Chains: A, B
Length: 317 amino acids
Theoretical weight: 35.24 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P65752 (Residues: 1-309; Coverage: 100%)
Gene names: SAV1919, ppaC
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P6122
Unit cell:
a: 99.406Å b: 99.406Å c: 425.285Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.222 0.22 0.249
Expression system: Escherichia coli BL21(DE3)