4roe

X-ray diffraction
2.2Å resolution

Human TFIIB-related factor 2 (Brf2) and TBP bound to RPPH1 promoter

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Transcription factor IIIB 50 kDa subunit Chain: A
Molecule details ›
Chain: A
Length: 360 amino acids
Theoretical weight: 40.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HAW0 (Residues: 62-419; Coverage: 85%)
Gene names: BRF2, BRFU, PRO1470
Structure domains: Cyclin-like
TATA-box-binding protein Chain: B
Molecule details ›
Chain: B
Length: 183 amino acids
Theoretical weight: 20.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P20226 (Residues: 159-339; Coverage: 53%)
Gene names: GTF2D1, TBP, TF2D, TFIID
Sequence domains: Transcription factor TFIID (or TATA-binding protein, TBP)
Structure domains: TATA-Binding Protein
Template strand Chain: N
Molecule details ›
Chain: N
Length: 28 nucleotides
Theoretical weight: 8.53 KDa
Non-template strand Chain: T
Molecule details ›
Chain: T
Length: 28 nucleotides
Theoretical weight: 8.68 KDa

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 77.741Å b: 91.926Å c: 102.423Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.192 0.222
Expression system: Escherichia coli