4rkb

X-ray diffraction
1.88Å resolution

Crystal structure of Staphylococcal nuclease variant Delta+PHS V66T/V99T at cryogenic temperature

Released:
Source organism: Staphylococcus aureus
Entry authors: Caro JA, Schlessman JL, Garcia-Moreno E B

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nuclease A Chain: A
Molecule details ›
Chain: A
Length: 143 amino acids
Theoretical weight: 16.15 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00644 (Residues: 83-231; Coverage: 70%)
Gene name: nuc
Sequence domains: Staphylococcal nuclease homologue
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P21
Unit cell:
a: 30.975Å b: 60.544Å c: 37.948Å
α: 90° β: 93.1° γ: 90°
R-values:
R R work R free
0.187 0.185 0.225
Expression system: Escherichia coli BL21(DE3)