› 4rk0

X-ray diffraction
1.8Å resolution

Crystal structure of LacI family transcriptional regulator from Enterococcus faecalis V583, Target EFI-512923, with bound ribose

Released:
DOI: 10.2210/pdb4rk0/pdb
PDB 4rk0 coloured by chain and viewed from the front.
PDB 4rk0 coloured by chain and viewed from the side.
PDB 4rk0 coloured by chain and viewed from the top.
Source organism: Enterococcus faecalis V583
Entry authors: Patskovsky Y, Toro R, Bhosle R, Al Obaidi N, Chamala S, Attonito JD, Scott Glenn A, Chowdhury S, Lafleur J, Siedel RD, Hillerich B, Love J, Whalen KL, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sugar-binding transcriptional regulator, LacI family Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 274 amino acids
Theoretical weight: 30.82 KDa
Source organism: Enterococcus faecalis V583
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q82ZT5 (Residues: 59-331; Coverage: 83%)
Gene name: EF_2962
Sequence domains: Periplasmic binding protein-like domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand RIB 4 x RIB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P1
Unit cell:
a: 36.919Å b: 77.234Å c: 91.805Å
α: 87.72° β: 82.57° γ: 84.33°
R-values:
R R work R free
0.172 0.17 0.223
Expression system: Escherichia coli BL21(DE3)

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