› 4rjx

X-ray diffraction
1.54Å resolution

Crystal structure of the OprO mutant protein F62Y/D114Y

Released:
DOI: 10.2210/pdb4rjx/pdb
PDB 4rjx coloured by chain and viewed from the front.
PDB 4rjx coloured by chain and viewed from the side.
PDB 4rjx coloured by chain and viewed from the top.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Structure, Dynamics, and Substrate Specificity of the OprO Porin from Pseudomonas aeruginosa.
Modi N, Ganguly S, Bárcena-Uribarri I, Benz R, van den Berg B, Kleinekathöfer U
Biophys. J. 109 1429-38 (2015)
PMID: 26445443

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Porin O Chain: A
Molecule details ›
Chain: A
Length: 429 amino acids
Theoretical weight: 47.35 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: P32977 (Residues: 25-438; Coverage: 100%)
Gene names: PA3280, oprO
Sequence domains: Phosphate-selective porin O and P
Structure domains: Porin

Ligands and Environments

2 bound ligands:
Ligand C8E 4 x C8E
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: R3
Unit cell:
a: 87.479Å b: 87.479Å c: 159.04Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.156 0.154 0.179
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Bacterial Resistance to Antimicrobial Agents.
Varela et al. (2021)
3 more

1 mention without citation

Conversion of OprO into an OprP-like Channel by Exchanging Key Residues in the Channel Constriction.
Ganguly et al. (2017)