X-ray diffraction
3.3Å resolution

Human FAN1 nuclease


Function and Biology Details

Reaction catalysed:
Hydrolytically removes 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Entry contents:
1 distinct polypeptide molecule
Fanconi-associated nuclease 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 640 amino acids
Theoretical weight: 72.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q9Y2M0 (Residues: 370-1009; Coverage: 63%)
Gene names: FAN1, KIAA1018, MTMR15
Sequence domains: VRR-NUC domain

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2221
Unit cell:
a: 146.882Å b: 156.885Å c: 205.396Å
α: 90° β: 90° γ: 90°
R R work R free
0.247 0.246 0.288
Expression system: Escherichia coli BL21(DE3)