4ri7 › Citations

The poplar Phi class glutathione transferase: expression, activity and structure of GSTF1.

Pégeot H, Koh CS, Petre B, Mathiot S, Duplessis S, Hecker A, Didierjean C, Rouhier N
Front Plant Sci 5 712 (2014)
Cited: 22 times
EuropePMC logo PMID: 25566286

Abstract

Glutathione transferases (GSTs) constitute a superfamily of enzymes with essential roles in cellular detoxification and secondary metabolism in plants as in other organisms. Several plant GSTs, including those of the Phi class (GSTFs), require a conserved catalytic serine residue to perform glutathione (GSH)-conjugation reactions. Genomic analyses revealed that terrestrial plants have around ten GSTFs, eight in the Populus trichocarpa genome, but their physiological functions and substrates are mostly unknown. Transcript expression analyses showed a predominant expression of all genes both in reproductive (female flowers, fruits, floral buds) and vegetative organs (leaves, petioles). Here, we show that the recombinant poplar GSTF1 (PttGSTF1) possesses peroxidase activity toward cumene hydroperoxide and GSH-conjugation activity toward model substrates such as 2,4-dinitrochlorobenzene, benzyl and phenetyl isothiocyanate, 4-nitrophenyl butyrate and 4-hydroxy-2-nonenal but interestingly not on previously identified GSTF-class substrates. In accordance with analytical gel filtration data, crystal structure of PttGSTF1 showed a canonical dimeric organization with bound GSH or 2-(N-morpholino)ethanesulfonic acid molecules. The structure of these protein-substrate complexes allowed delineating the residues contributing to both the G and H sites that form the active site cavity. In sum, the presence of GSTF1 transcripts and proteins in most poplar organs especially those rich in secondary metabolites such as flowers and fruits, together with its GSH-conjugation activity and its documented stress-responsive expression suggest that its function is associated with the catalytic transformation of metabolites and/or peroxide removal rather than with ligandin properties as previously reported for other GSTFs.

Reviews - 4ri7 mentioned but not cited (1)

  1. Functional, Structural and Biochemical Features of Plant Serinyl-Glutathione Transferases. Sylvestre-Gonon E, Law SR, Schwartz M, Robe K, Keech O, Didierjean C, Dubos C, Rouhier N, Hecker A. Front Plant Sci 10 608 (2019)

Articles - 4ri7 mentioned but not cited (2)

  1. The poplar Phi class glutathione transferase: expression, activity and structure of GSTF1. Pégeot H, Koh CS, Petre B, Mathiot S, Duplessis S, Hecker A, Didierjean C, Rouhier N. Front Plant Sci 5 712 (2014)
  2. Robust AMBER Force Field Parameters for Glutathionylated Cysteines. Elftmaoui Z, Bignon E. Int J Mol Sci 24 15022 (2023)


Reviews citing this publication (1)

  1. Glutathione S-transferase: a versatile protein family. Vaish S, Gupta D, Mehrotra R, Mehrotra S, Basantani MK. 3 Biotech 10 321 (2020)

Articles citing this publication (18)

  1. Candidate Effector Proteins of the Rust Pathogen Melampsora larici-populina Target Diverse Plant Cell Compartments. Petre B, Saunders DG, Sklenar J, Lorrain C, Win J, Duplessis S, Kamoun S. Mol Plant Microbe Interact 28 689-700 (2015)
  2. Differential Roles for VviGST1, VviGST3, and VviGST4 in Proanthocyanidin and Anthocyanin Transport in Vitis vinífera. Pérez-Díaz R, Madrid-Espinoza J, Salinas-Cornejo J, González-Villanueva E, Ruiz-Lara S. Front Plant Sci 7 1166 (2016)
  3. Three Camellia sinensis glutathione S-transferases are involved in the storage of anthocyanins, flavonols, and proanthocyanidins. Liu Y, Jiang H, Zhao Y, Li X, Dai X, Zhuang J, Zhu M, Jiang X, Wang P, Gao L, Xia T. Planta 250 1163-1175 (2019)
  4. Structural evidence for Arabidopsis glutathione transferase AtGSTF2 functioning as a transporter of small organic ligands. Ahmad L, Rylott EL, Bruce NC, Edwards R, Grogan G. FEBS Open Bio 7 122-132 (2017)
  5. Evolutionary divergence of Ure2pA glutathione transferases in wood degrading fungi. Roret T, Thuillier A, Favier F, Gelhaye E, Didierjean C, Morel-Rouhier M. Fungal Genet Biol 83 103-112 (2015)
  6. Structural plasticity among glutathione transferase Phi members: natural combination of catalytic residues confers dual biochemical activities. Pégeot H, Mathiot S, Perrot T, Gense F, Hecker A, Didierjean C, Rouhier N. FEBS J 284 2442-2463 (2017)
  7. Methylglyoxal Detoxification Revisited: Role of Glutathione Transferase in Model Cyanobacterium Synechocystis sp. Strain PCC 6803. Kammerscheit X, Hecker A, Rouhier N, Chauvat F, Cassier-Chauvat C. mBio 11 e00882-20 (2020)
  8. Expression of barley Glutathione S-Transferase13 gene reduces accumulation of reactive oxygen species by trichothecenes and paraquat in Arabidopsis plants. Wahibah NN, Tsutsui T, Tamaoki D, Sato K, Nishiuchi T. Plant Biotechnol (Tokyo) 35 71-79 (2018)
  9. Expanding the Plant GSTome Through Directed Evolution: DNA Shuffling for the Generation of New Synthetic Enzymes With Engineered Catalytic and Binding Properties. Chronopoulou EG, Papageorgiou AC, Ataya F, Nianiou-Obeidat I, Madesis P, Labrou NE. Front Plant Sci 9 1737 (2018)
  10. Phi Class of Glutathione S-transferase Gene Superfamily Widely Exists in Nonplant Taxonomic Groups. Munyampundu JP, Xu YP, Cai XZ. Evol Bioinform Online 12 59-71 (2016)
  11. The Poplar Rust-Induced Secreted Protein (RISP) Inhibits the Growth of the Leaf Rust Pathogen Melampsora larici-populina and Triggers Cell Culture Alkalinisation. Petre B, Hecker A, Germain H, Tsan P, Sklenar J, Pelletier G, Séguin A, Duplessis S, Rouhier N. Front Plant Sci 7 97 (2016)
  12. The Glutathione S-Transferase PtGSTF1 Improves Biomass Production and Salt Tolerance through Regulating Xylem Cell Proliferation, Ion Homeostasis and Reactive Oxygen Species Scavenging in Poplar. Gao H, Yu C, Liu R, Li X, Huang H, Wang X, Zhang C, Jiang N, Li X, Cheng S, Zhang H, Li B. Int J Mol Sci 23 11288 (2022)
  13. Biochemical and Structural Characterization of Chi-Class Glutathione Transferases: A Snapshot on the Glutathione Transferase Encoded by sll0067 Gene in the Cyanobacterium Synechocystis sp. Strain PCC 6803. Mocchetti E, Morette L, Mulliert G, Mathiot S, Guillot B, Dehez F, Chauvat F, Cassier-Chauvat C, Brochier-Armanet C, Didierjean C, Hecker A. Biomolecules 12 1466 (2022)
  14. Biochemical and Structural Insights on the Poplar Tau Glutathione Transferase GSTU19 and 20 Paralogs Binding Flavonoids. Sylvestre-Gonon E, Morette L, Viloria M, Mathiot S, Boutilliat A, Favier F, Rouhier N, Didierjean C, Hecker A. Front Mol Biosci 9 958586 (2022)
  15. Cross-stress gene expression atlas of Marchantia polymorpha reveals the hierarchy and regulatory principles of abiotic stress responses. Tan QW, Lim PK, Chen Z, Pasha A, Provart N, Arend M, Nikoloski Z, Mutwil M. Nat Commun 14 986 (2023)
  16. Directed Evolution of Phi Class Glutathione Transferases Involved in Multiple-Herbicide Resistance of Grass Weeds and Crops. Ioannou E, Papageorgiou AC, Labrou NE. Int J Mol Sci 23 7469 (2022)
  17. A Key Role in Catalysis and Enzyme Thermostability of a Conserved Helix H5 Motif of Human Glutathione Transferase A1-1. Chronopoulou EG, Mutabdzija L, Poudel N, Papageorgiou AC, Labrou NE. Int J Mol Sci 24 3700 (2023)
  18. Genes encoding cytochrome P450 monooxygenases and glutathione S-transferases associated with herbicide resistance evolved before the origin of land plants. Casey A, Dolan L. PLoS One 18 e0273594 (2023)


Quick links