› 4ri7

X-ray diffraction
1.8Å resolution

Crystal structure of poplar glutathione transferase F1 mutant SER 13 CYS

Released:
DOI: 10.2210/pdb4ri7/pdb
PDB entry 4ri7 coloured by chain, front view.
PDB entry 4ri7 coloured by chain, side view.
PDB entry 4ri7 coloured by chain, top view.
Source organism: Populus tremula x Populus tremuloides
Primary publication:
The poplar Phi class glutathione transferase: expression, activity and structure of GSTF1.
Pégeot H, Koh CS, Petre B, Mathiot S, Duplessis S, Hecker A, Didierjean C, Rouhier N
Front Plant Sci 5 712 (2014)
PMID: 25566286

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109028 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
glutathione transferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 215 amino acids
Theoretical weight: 24.37 KDa
Source organism: Populus tremula x Populus tremuloides
Expression system: Escherichia coli
UniProt:
  • Canonical: A9PHH6 (Residues: 1-215; Coverage: 100%)
Gene name: POPTR_002G015100
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GSH 2 x GSH
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P212121
Unit cell:
a: 55.299Å b: 60.649Å c: 119.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.15 0.195
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Glutathione S-transferase: a versatile protein family.
Vaish et al. (2020)
18 other articles

3 mentions without citation

Functional, Structural and Biochemical Features of Plant Serinyl-Glutathione Transferases.
Sylvestre-Gonon et al. (2019)
2 more