› 4rhh

X-ray diffraction
2.15Å resolution

Crystal structure of the catalytic mutant Xyn52B2-E335G, a GH52 Beta-D-xylosidase from Geobacillus stearothermophilus T6

Released:
DOI: 10.2210/pdb4rhh/pdb
PDB 4rhh coloured by chain and viewed from the front.
PDB 4rhh coloured by chain and viewed from the side.
PDB 4rhh coloured by chain and viewed from the top.
Source organism: Geobacillus stearothermophilus
Entry authors: Dann R, Lansky S, Lavid N, Zehavi A, Belakhov V, Baasov T, Manjasetty B, Belrhali H, Shoham Y, Shoham G

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-xylosidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 705 amino acids
Theoretical weight: 79.91 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q09LZ0 (Residues: 1-705; Coverage: 100%)
Gene name: xynB2
Sequence domains: Glycosyl hydrolase family 52

Ligands and Environments

2 bound ligands:
Ligand CA 5 x CA
Ligand TRS 7 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 97.45Å b: 119.08Å c: 243.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.15 0.203
Expression system: Escherichia coli

Quick links