4rhh

X-ray diffraction
2.15Å resolution

Crystal structure of the catalytic mutant Xyn52B2-E335G, a GH52 Beta-D-xylosidase from Geobacillus stearothermophilus T6

Released:
Entry authors: Dann R, Lansky S, Lavid N, Zehavi A, Belakhov V, Baasov T, Manjasetty B, Belrhali H, Shoham Y, Shoham G

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-xylosidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 705 amino acids
Theoretical weight: 79.91 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q09LZ0 (Residues: 1-705; Coverage: 100%)
Gene name: xynB2
Sequence domains: Glycosyl hydrolase family 52

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P212121
Unit cell:
a: 97.45Å b: 119.08Å c: 243.02Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.153 0.15 0.203
Expression system: Escherichia coli