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Crystal structure of the in-line aligned env22 twister ribozyme

X-ray diffraction
2.89Å resolution
PDB entry 4rge coloured by chain, front view.
PDB entry 4rge coloured by chain, side view.
PDB entry 4rge coloured by chain, top view.
Source organism: synthetic construct
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct RNA molecule

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Assemblies

Assembly 1
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Assembly Name: RNA
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Multimeric state: monomeric
Accessible surface area: 8812.65 Å2
Buried surface area: 917.6 Å2
Dissociation area: 34.1 Å2
Dissociation energy (ΔGdiss): 5.76 kcal/mol
Dissociation entropy (TΔSdiss): -1.47 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-196869
Assembly 2 (preferred)
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Assembly Name: RNA
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Multimeric state: monomeric
Accessible surface area: 8693.67 Å2
Buried surface area: 830.24 Å2
Dissociation area: 52.69 Å2
Dissociation energy (ΔGdiss): 7.2 kcal/mol
Dissociation entropy (TΔSdiss): -1.46 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-196869
Assembly 3
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Assembly Name: RNA
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Multimeric state: monomeric
Accessible surface area: 8548.43 Å2
Buried surface area: 887.88 Å2
Dissociation area: 36.88 Å2
Dissociation energy (ΔGdiss): 6.12 kcal/mol
Dissociation entropy (TΔSdiss): -1.47 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-196869

Macromolecules

RNA chains A, B, C
Name: env22 twister ribozyme
Representative chains: A, B, C
Source organism: synthetic construct [32630]
Expression system: Not provided
Length: 56 nucleotides
Theoretical weight: 18.01 KDa
env22 twister ribozyme in PDB entry 4rge, assembly 2, front view.
env22 twister ribozyme in PDB entry 4rge, assembly 2, side view.
env22 twister ribozyme in PDB entry 4rge, assembly 2, top view.
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