X-ray diffraction
1.5Å resolution

Complex structure of AlkB/rhein

Source organism: Escherichia coli
Primary publication:
Rhein Inhibits AlkB Repair Enzymes and Sensitizes Cells to Methylated DNA Damage.
J. Biol. Chem. 291 11083-93 (2016)
PMID: 27015802

Function and Biology Details

Reaction catalysed:
DNA-base-CH(3) + 2-oxoglutarate + O(2) = DNA-base + formaldehyde + succinate + CO(2)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Alpha-ketoglutarate-dependent dioxygenase AlkB Chain: B
Molecule details ›
Chain: B
Length: 203 amino acids
Theoretical weight: 22.56 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
  • Canonical: P05050 (Residues: 14-216; Coverage: 94%)
Gene names: JW2200, aidD, alkB, b2212
Structure domains: Alpha-ketoglutarate-dependent dioxygenase AlkB-like

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P1
Unit cell:
a: 36.95Å b: 39.2Å c: 40.521Å
α: 77.37° β: 74.67° γ: 65.47°
R R work R free
0.119 0.118 0.15
Expression system: Escherichia coli