PDBe 4rew

X-ray diffraction
4.58Å resolution

Crystal structure of the non-phosphorylated human alpha1 beta2 gamma1 holo-AMPK complex

Released:

Function and Biology Details

Reactions catalysed:
ATP + [tau protein] = ADP + [tau protein] phosphate
ATP + [acetyl-CoA carboxylase] = ADP + [acetyl-CoA carboxylase] phosphate
ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)] = ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
ATP + a protein = ADP + a phosphoprotein
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
5'-AMP-activated protein kinase catalytic subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 540 amino acids
Theoretical weight: 61.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13131 (Residues: 20-559; Coverage: 97%)
Gene names: AMPK1, PRKAA1
Sequence domains:
5'-AMP-activated protein kinase subunit beta-2 Chain: B
Molecule details ›
Chain: B
Length: 197 amino acids
Theoretical weight: 22.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O43741 (Residues: 76-272; Coverage: 72%)
Gene name: PRKAB2
Sequence domains:
5'-AMP-activated protein kinase subunit gamma-1 Chain: G
Molecule details ›
Chain: G
Length: 304 amino acids
Theoretical weight: 34.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P54619 (Residues: 24-327; Coverage: 92%)
Gene name: PRKAG1
Sequence domains: CBS domain

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P3221
Unit cell:
a: 126.9Å b: 126.9Å c: 188.794Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.234 0.232 0.259
Expression system: Escherichia coli BL21(DE3)