4rep

X-ray diffraction
1.97Å resolution

Crystal Structure of gamma-carotenoid desaturase

Released:
Source organism: Nonlabens dokdonensis DSW-6
Primary publication:
Crystal structure of 1'-OH-carotenoid 3,4-desaturase from Nonlabens dokdonensis DSW-6.
Enzyme Microb. Technol. 77 29-37 (2015)
PMID: 26138397

Function and Biology Details

Reaction catalysed:
1-hydroxy-1,2-dihydrolycopene + acceptor = 1-hydroxy-3,4-didehydro-1,2-dihydrolycopene + reduced acceptor
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-hydroxycarotenoid 3,4-desaturase Chain: A
Molecule details ›
Chain: A
Length: 495 amino acids
Theoretical weight: 55.91 KDa
Source organism: Nonlabens dokdonensis DSW-6
Expression system: Escherichia coli
UniProt:
  • Canonical: L7WC64 (Residues: 1-487; Coverage: 100%)
Gene names: DDD_2394, crtD
Sequence domains: Flavin containing amine oxidoreductase

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 7A (6B, 6C1), SPRING-8 BEAMLINE BL26B1
Spacegroup: P21
Unit cell:
a: 48.369Å b: 72.835Å c: 68.137Å
α: 90° β: 110.29° γ: 90°
R-values:
R R work R free
0.163 0.16 0.224
Expression system: Escherichia coli