X-ray diffraction
0.74Å resolution

Crystal structure and charge density studies of cholesterol oxidase from Brevibacterium sterolicum at 0.74 ultra-high resolution


Function and Biology Details

Reactions catalysed:
Cholesterol + O(2) = cholest-5-en-3-one + H(2)O(2)
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Cholesterol oxidase Chain: A
Molecule details ›
Chain: A
Length: 499 amino acids
Theoretical weight: 54.53 KDa
Source organism: Streptomyces sp. SA-COO
Expression system: Escherichia coli
  • Canonical: P12676 (Residues: 46-544; Coverage: 99%)
Gene name: choA
Sequence domains:
Structure domains:

Ligands and Environments

Cofactor: Ligand FAD 1 x FAD
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 51.24Å b: 72.92Å c: 63.01Å
α: 90° β: 105.13° γ: 90°
R R work R free
0.113 0.113 0.123
Expression system: Escherichia coli