› 4re0

X-ray diffraction
2.35Å resolution

Crystal structure of VmoLac in P622 space group

Released:
DOI: 10.2210/pdb4re0/pdb
PDB entry 4re0 coloured by chain, front view.
PDB entry 4re0 coloured by chain, side view.
PDB entry 4re0 coloured by chain, top view.
Source organism: Vulcanisaeta moutnovskia 768-28
Primary publication:
Crystal structure of VmoLac, a tentative quorum quenching lactonase from the extremophilic crenarchaeon Vulcanisaeta moutnovskia.
Hiblot J, Bzdrenga J, Champion C, Chabriere E, Elias M
Sci Rep 5 8372 (2015)
PMID: 25670483

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123265 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Parathion hydrolase Chain: A
Molecule details ›
Chain: A
Length: 316 amino acids
Theoretical weight: 35.6 KDa
Source organism: Vulcanisaeta moutnovskia 768-28
Expression system: Escherichia coli
UniProt:
  • Canonical: F0QXN6 (Residues: 1-315; Coverage: 100%)
Gene name: VMUT_2255
Sequence domains: Phosphotriesterase family
Structure domains: Metal-dependent hydrolases

Ligands and Environments

4 bound ligands:
Ligand CO 2 x CO
Ligand GOL 4 x GOL
Ligand MYR 1 x MYR
Ligand SO4 2 x SO4
1 modified residue:
Ligand KCX 1 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P6122
Unit cell:
a: 134.67Å b: 134.67Å c: 126.4Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.141 0.14 0.17
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Biotechnological applications of quorum quenching enzymes.
Bzdrenga et al. (2017)
5 more

1 mention without citation

Crystal structure of VmoLac, a tentative quorum quenching lactonase from the extremophilic crenarchaeon Vulcanisaeta moutnovskia.
Hiblot et al. (2015)