4rbo

X-ray diffraction
3.3Å resolution

Crystal structure of a Nanog homeobox (NANOG) from Homo sapiens at 3.30 A resolution

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Homeobox protein NANOG Chains: A, D
Molecule details ›
Chains: A, D
Length: 70 amino acids
Theoretical weight: 8.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H9S0 (Residues: 94-162; Coverage: 23%)
Gene name: NANOG
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
5'-D(*GP*GP*CP*CP*CP*AP*TP*TP*CP*AP*AP*G)-3' Chains: B, E
Molecule details ›
Chains: B, E
Length: 12 nucleotides
Theoretical weight: 3.65 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(*CP*TP*TP*GP*AP*AP*TP*GP*GP*GP*CP*C)-3' Chains: C, F
Molecule details ›
Chains: C, F
Length: 12 nucleotides
Theoretical weight: 3.68 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P6522
Unit cell:
a: 215.603Å b: 215.603Å c: 42.269Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.236 0.235 0.268
Expression systems:
  • Escherichia coli
  • Not provided