› 4raz

X-ray diffraction
1.9Å resolution

Crystal structure of Magnetospirillum gryphiswaldense MSR-1 holo-Fur

Released:
DOI: 10.2210/pdb4raz/pdb
PDB 4raz coloured by chain and viewed from the front.
PDB 4raz coloured by chain and viewed from the side.
PDB 4raz coloured by chain and viewed from the top.
Source organism: Magnetospirillum gryphiswaldense MSR-1 v2
Primary publication:
Mechanistic insights into metal ion activation and operator recognition by the ferric uptake regulator.
Deng Z, Wang Q, Liu Z, Zhang M, Machado AC, Chiu TP, Feng C, Zhang Q, Yu L, Qi L, Zheng J, Wang X, Huo X, Qi X, Li X, Wu W, Rohs R, Li Y, Chen Z
OpenAccess logo Nat Commun 6 7642 (2015)
PMID: 26134419

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferric uptake regulation protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 145 amino acids
Theoretical weight: 16.63 KDa
Source organism: Magnetospirillum gryphiswaldense MSR-1 v2
Expression system: Escherichia coli
UniProt:
  • Canonical: V6F4Q0 (Residues: 1-143; Coverage: 100%)
Gene names: Fur, MGMSRv2__3137, fur
Sequence domains: Ferric uptake regulator family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MN 4 x MN
Ligand EDO 3 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: I222
Unit cell:
a: 79.595Å b: 96.847Å c: 119.874Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.203 0.227
Expression system: Escherichia coli

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Citations

7 review citations

Allosteric control of metal-responsive transcriptional regulators in bacteria.
Baksh et al. (2020)
6 more

1 mention without citation

Redox Sensing by Fe2+ in Bacterial Fur Family Metalloregulators.
Pinochet-Barros et al. (2018)