4rau

X-ray diffraction
3.74Å resolution

crystal structure of RTOFab in complex with human PF4

Released:
Source organisms:
Entry authors: Cai Z, Zhu Z, Liu Q, Greene MI

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
RTOFab light chain Chains: A, D, G, J, M, P, S, V
Molecule details ›
Chains: A, D, G, J, M, P, S, V
Length: 212 amino acids
Theoretical weight: 23.38 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
RTOFab heavy chain Chains: B, E, H, K, N, Q, T, W
Molecule details ›
Chains: B, E, H, K, N, Q, T, W
Length: 219 amino acids
Theoretical weight: 23.65 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Platelet factor 4 Chains: C, F, I, L, O, R, U, X
Molecule details ›
Chains: C, F, I, L, O, R, U, X
Length: 70 amino acids
Theoretical weight: 7.78 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P02776 (Residues: 32-101; Coverage: 100%)
Gene names: CXCL4, PF4, SCYB4
Sequence domains: Small cytokines (intecrine/chemokine), interleukin-8 like
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21212
Unit cell:
a: 161.424Å b: 171.873Å c: 208.159Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.243 0.284
Expression systems:
  • Mus musculus
  • Drosophila melanogaster