› 4r9v

X-ray diffraction
2.3Å resolution

Crystal structure of sialyltransferase from photobacterium damselae, residues 113-497 corresponding to the gt-b domain

Released:
DOI: 10.2210/pdb4r9v/pdb
PDB entry 4r9v coloured by chain, front view.
PDB entry 4r9v coloured by chain, side view.
PDB entry 4r9v coloured by chain, top view.
Source organism: Photobacterium damselae
Primary publication:
Crystal structures of sialyltransferase from Photobacterium damselae.
Huynh N, Li Y, Yu H, Huang S, Lau K, Chen X, Fisher AJ
FEBS Lett 588 4720-9 (2014)
PMID: 25451227

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130302 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PhoU domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 406 amino acids
Theoretical weight: 46.12 KDa
Source organism: Photobacterium damselae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O66375 (Residues: 113-497; Coverage: 57%)
Gene name: bst
Sequence domains: Sialyltransferase PMO188
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: C2
Unit cell:
a: 95.444Å b: 56.523Å c: 87.375Å
α: 90° β: 119.95° γ: 90°
R-values:
R R work R free
0.187 0.184 0.248
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Glycosyltransferases: mechanisms and applications in natural product development.
Liang et al. (2015)
1 more

2 mentions without citation

Cellular and Molecular Engineering of Glycan Sialylation in Heterologous Systems.
Hombu et al. (2021)
1 more