4r8w

X-ray diffraction
2.8Å resolution

Crystal structure of H7 hemagglutinin from A/Anhui/1/2013 in complex with a neutralizing antibody CT149

Released:
Entry authors: Wu Y, Shi Y, Qi J, Gao GF

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Hemagglutinin Chain: A
Molecule details ›
Chain: A
Length: 321 amino acids
Theoretical weight: 34.99 KDa
Source organism: Influenza A virus (A/Anhui/1-BALF_RG45/2013(H7N9))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: A0A024E3P0 (Residues: 19-339; Coverage: 57%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains:
Hemagglutinin Chain: B
Molecule details ›
Chain: B
Length: 177 amino acids
Theoretical weight: 20.44 KDa
Source organism: Influenza A virus (A/Anhui/1-BALF_RG45/2013(H7N9))
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: A0A024E2M1 (Residues: 340-516; Coverage: 32%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin Ectodomain; Chain B
Heavy chain of neutralizing antibody CT149 Chain: H
Molecule details ›
Chain: H
Length: 231 amino acids
Theoretical weight: 24.43 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Structure domains: Immunoglobulins
Light chain of neutralizing antibody CT149 Chain: L
Molecule details ›
Chain: L
Length: 216 amino acids
Theoretical weight: 23.76 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: R32
Unit cell:
a: 126.856Å b: 126.856Å c: 409.596Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.265 0.262 0.311
Expression system: Spodoptera frugiperda