4r8q

X-ray diffraction
2.31Å resolution

Structure and substrate recruitment of the human spindle checkpoint kinase bub1

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitotic checkpoint serine/threonine-protein kinase BUB1 Chain: A
Molecule details ›
Chain: A
Length: 365 amino acids
Theoretical weight: 41.85 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O43683 (Residues: 724-1085; Coverage: 33%)
Gene names: BUB1, BUB1L
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 108.768Å b: 147.632Å c: 47.196Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.222 0.264
Expression system: Spodoptera frugiperda