› 4r7u

X-ray diffraction
2.45Å resolution

Structure of UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Vibrio cholerae in complex with substrate UDP-N-acetylglucosamine and the drug fosfomycin

Released:
DOI: 10.2210/pdb4r7u/pdb
PDB 4r7u coloured by chain and viewed from the front.
PDB 4r7u coloured by chain and viewed from the side.
PDB 4r7u coloured by chain and viewed from the top.
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Entry authors: Nocek B, Maltseva N, Anderson W, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylglucosamine 1-carboxyvinyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 422 amino acids
Theoretical weight: 45.01 KDa
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KP62 (Residues: 1-419; Coverage: 100%)
Gene names: VC_2514, murA
Sequence domains: EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Structure domains: Enolpyruvate transferase domain

Ligands and Environments

4 bound ligands:
Ligand PG4 1 x PG4
Ligand FFQ 4 x FFQ
Ligand UD1 4 x UD1
Ligand NA 5 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: R3
Unit cell:
a: 204.283Å b: 204.283Å c: 118.878Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.18 0.178 0.234
Expression system: Escherichia coli BL21(DE3)

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