› 4r70

X-ray diffraction
2.85Å resolution

Crystal structure of bacteriophytochrome RpBphP3 from photosynthetic bacterium R. palustris

Released:
DOI: 10.2210/pdb4r70/pdb
PDB entry 4r70 coloured by chain, front view.
PDB entry 4r70 coloured by chain, side view.
PDB entry 4r70 coloured by chain, top view.
Source organism: Rhodopseudomonas palustris CGA009
Primary publication:
Light Signaling Mechanism of Two Tandem Bacteriophytochromes.
Yang X, Stojković EA, Ozarowski WB, Kuk J, Davydova E, Moffat K
Structure 23 1179-89 (2015)
PMID: 26095026

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-179962 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
histidine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 529 amino acids
Theoretical weight: 58.82 KDa
Source organism: Rhodopseudomonas palustris CGA009
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6N5G2 (Residues: 1-521; Coverage: 67%)
Gene names: RPA3016, TX73_015620, phyB2
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand BLA 2 x BLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P3121
Unit cell:
a: 143.307Å b: 143.307Å c: 120.512Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.186 0.26
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Bacteriophytochromes - from informative model systems of phytochrome function to powerful tools in cell biology.
Gourinchas et al. (2019)
2 more

3 mentions without citation

Light Signaling Mechanism of Two Tandem Bacteriophytochromes.
Yang et al. (2015)
2 more