X-ray diffraction
1.22Å resolution

Crystal structure of solute-binding protein stm0429 from salmonella enterica subsp. enterica serovar typhimurium str. lt2, target efi-510776, a closed conformation, in complex with glycerol and acetate

Entry authors: Patskovsky Y, Toro R, Bhosle R, Al obaidi N, Chamala S, Attonito JD, Scott glenn A, Chowdhury S, Lafleur J, Siedel RD, Hillerich B, Love J, Whalen KL, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Putative 2-aminoethylphosphonate-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 318 amino acids
Theoretical weight: 34.58 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P96062 (Residues: 22-337; Coverage: 100%)
Gene names: STM0429, phnS
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 55.185Å b: 67.601Å c: 77.36Å
α: 90° β: 90° γ: 90°
R R work R free
0.115 0.114 0.148
Expression system: Escherichia coli BL21(DE3)