› 4r4q

X-ray diffraction
1.35Å resolution

Crystal structure of RPA70N in complex with 5-(3-((N-(4-(5-carboxyfuran-2-yl)benzyl)acetamido)methyl)phenyl)-1-(3,4-dichlorophenyl)-1H-pyrazole-3-carboxylic acid

Released:
DOI: 10.2210/pdb4r4q/pdb
PDB 4r4q coloured by chain and viewed from the front.
PDB 4r4q coloured by chain and viewed from the side.
PDB 4r4q coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Diphenylpyrazoles as replication protein a inhibitors.
Waterson AG, Kennedy JP, Patrone JD, Pelz NF, Feldkamp MD, Frank AO, Vangamudi B, Souza-Fagundes EM, Rossanese OW, Chazin WJ, Fesik SW
ACS Med Chem Lett 6 140-5 (2015)
PMID: 25699140

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Replication protein A 70 kDa DNA-binding subunit, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 123 amino acids
Theoretical weight: 13.5 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P27694 (Residues: 1-120; Coverage: 20%)
Gene names: REPA1, RPA1, RPA70
Sequence domains: Replication factor-A protein 1, N-terminal domain
Structure domains: Nucleic acid-binding proteins

Ligands and Environments

1 bound ligand:
Ligand 3HZ 1 x 3HZ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 38.161Å b: 53.934Å c: 53.975Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.15 0.148 0.188
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

OB-Folds and Genome Maintenance: Targeting Protein-DNA Interactions for Cancer Therapy.
Par et al. (2021)
3 more

5 mentions without citation

Sodium channel activation gating is affected by substitutions of voltage sensor positive charges in all four domains.
Kontis et al. (1997)
4 more