› 4r39

X-ray diffraction
2.6Å resolution

Histidine kinase domain from Erythrobacter litoralis EL346 blue-light activated histidine kinase

Released:
DOI: 10.2210/pdb4r39/pdb
PDB entry 4r39 coloured by chain, front view.
PDB entry 4r39 coloured by chain, side view.
PDB entry 4r39 coloured by chain, top view.
Source organism: Erythrobacter litoralis HTCC2594
Primary publication:
Full-length structure of a monomeric histidine kinase reveals basis for sensory regulation.
Rivera-Cancel G, Ko WH, Tomchick DR, Correa F, Gardner KH
Proc Natl Acad Sci U S A 111 17839-44 (2014)
PMID: 25468971

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173850 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Blue-light-activated histidine kinase 2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 232 amino acids
Theoretical weight: 24.93 KDa
Source organism: Erythrobacter litoralis HTCC2594
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q2NB77 (Residues: 121-346; Coverage: 65%)
Gene name: ELI_04860
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand ANP 4 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 120.503Å b: 120.503Å c: 192.304Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.221
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

Molecular Mechanisms of Two-Component Signal Transduction.
Zschiedrich et al. (2016)
18 more

3 mentions without citation

Full-length structure of a monomeric histidine kinase reveals basis for sensory regulation.
Rivera-Cancel et al. (2014)
2 more