X-ray diffraction
2Å resolution

Crystal structure of a putative uridine phosphorylase from Actinobacillus succinogenes 130Z (Target NYSGRC-029667 )

Entry authors: Sampathkumar P, Almo SC, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed:
Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Uridine phosphorylase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 279 amino acids
Theoretical weight: 30.43 KDa
Source organism: Actinobacillus succinogenes 130Z
Expression system: Escherichia coli BL21(DE3)
  • Canonical: A6VLF7 (Residues: 1-257; Coverage: 100%)
Gene name: Asuc_0427
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P212121
Unit cell:
a: 86.422Å b: 120.595Å c: 176.879Å
α: 90° β: 90° γ: 90°
R R work R free
0.171 0.169 0.208
Expression system: Escherichia coli BL21(DE3)