4r2g

X-ray diffraction
3.28Å resolution

Crystal Structure of PGT124 Fab bound to HIV-1 JRCSF gp120 core and to CD4

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (6 distinct):
Surface protein gp120 Chains: A, E, K, O
Molecule details ›
Chains: A, E, K, O
Length: 309 amino acids
Theoretical weight: 34.75 KDa
Source organism: Human immunodeficiency virus type 1 (JRCSF ISOLATE)
Expression system: Homo sapiens
UniProt:
  • Canonical: P20871 (Residues: 197-300, 308-484; Coverage: 33%)
Gene name: env
Structure domains: Human immunodeficiency virus 1, Gp160, envelope glycoprotein
T-cell surface glycoprotein CD4 Chains: B, F, H, L
Molecule details ›
Chains: B, F, H, L
Length: 184 amino acids
Theoretical weight: 20.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01730 (Residues: 26-208; Coverage: 42%)
Gene name: CD4
Sequence domains: Immunoglobulin C2-set domain
Structure domains: Immunoglobulins
PGT124 Light Chain Chains: C, I, M, P
Molecule details ›
Chains: C, I, M, P
Length: 214 amino acids
Theoretical weight: 23.06 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
PGT124 Heavy Chain Chains: D, J, N, Q
Molecule details ›
Chains: D, J, N, Q
Length: 236 amino acids
Theoretical weight: 25.46 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer : NEW Components: NAG
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P212121
Unit cell:
a: 164.406Å b: 165.438Å c: 229.712Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.207 0.262
Expression systems:
  • Homo sapiens
  • Escherichia coli