4r22

X-ray diffraction
2.6Å resolution

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
HTH-type transcriptional regulator TnrA Chain: B
Molecule details ›
Chain: B
Length: 82 amino acids
Theoretical weight: 9.71 KDa
Source organism: Bacillus megaterium WSH-002
Expression system: Escherichia coli
UniProt:
  • Canonical: G2RUZ1 (Residues: 7-88; Coverage: 76%)
Gene names: BMWSH_3277, tnrA
Sequence domains: MerR HTH family regulatory protein
Structure domains: Multidrug-efflux Transporter Regulator; Chain: A; Domain 2
DNA (5'-D(*CP*GP*TP*GP*TP*AP*AP*GP*GP*AP*AP*TP*TP*CP*TP*GP*AP*CP*AP*CP*G)-3') Chain: G
Molecule details ›
Chain: G
Length: 21 nucleotides
Theoretical weight: 6.49 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21212
Unit cell:
a: 66.44Å b: 46.39Å c: 42.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.261 0.261 0.279
Expression systems:
  • Escherichia coli
  • Not provided