PDBe 4r1f

X-ray diffraction
2.51Å resolution

Re-refined Human DNA topoisomerase IIa (ATPase and transducer domains) in complex with ADP and SO4

Released:

Function and Biology Details

Reaction catalysed:
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA topoisomerase 2-alpha Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 400 amino acids
Theoretical weight: 45.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11388 (Residues: 29-428; Coverage: 26%)
Gene names: TOP2, TOP2A
Sequence domains: Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Structure domains:

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 71.015Å b: 90.492Å c: 148.293Å
α: 90° β: 89.97° γ: 90°
R-values:
R R work R free
0.211 0.208 0.243
Expression system: Escherichia coli