› 4r12

X-ray diffraction
1.95Å resolution

Crystal structure of the gamma-secretase component Nicastrin

Released:
DOI: 10.2210/pdb4r12/pdb
PDB entry 4r12 coloured by chain, front view.
PDB entry 4r12 coloured by chain, side view.
PDB entry 4r12 coloured by chain, top view.
Source organism: Dictyostelium purpureum
Primary publication:
Crystal structure of the γ-secretase component nicastrin.
Xie T, Yan C, Zhou R, Zhao Y, Sun L, Yang G, Lu P, Ma D, Shi Y
Proc Natl Acad Sci U S A 111 13349-54 (2014)
PMID: 25197054

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123280 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Nicastrin Chain: A
Molecule details ›
Chain: A
Length: 593 amino acids
Theoretical weight: 66.73 KDa
Source organism: Dictyostelium purpureum
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: F0ZBA6 (Residues: 19-611; Coverage: 95%)
Gene names: DICPUDRAFT_96800, ncstn
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
2 bound ligands:
Ligand NAG 3 x NAG
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P41212
Unit cell:
a: 65.584Å b: 65.584Å c: 344.475Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.179 0.206
Expression system: Spodoptera frugiperda

Quick links

Citations

11 review citations

NEURODEGENERATION. Alzheimer's and Parkinson's diseases: The prion concept in relation to assembled Aβ, tau, and α-synuclein.
Goedert M. (2015)
10 more

9 mentions without citation

Structural biology of presenilin 1 complexes.
Li et al. (2014)
8 more