› 4r0v

X-ray diffraction
2.29Å resolution

[FeFe]-hydrogenase Oxygen Inactivation is Initiated by the Modification and Degradation of the H cluster 2Fe Subcluster

Released:
DOI: 10.2210/pdb4r0v/pdb
PDB entry 4r0v coloured by chain, front view.
PDB entry 4r0v coloured by chain, side view.
PDB entry 4r0v coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
[FeFe]-hydrogenase oxygen inactivation is initiated at the H cluster 2Fe subcluster.
Swanson KD, Ratzloff MW, Mulder DW, Artz JH, Ghose S, Hoffman A, White S, Zadvornyy OA, Broderick JB, Bothner B, King PW, Peters JW
J Am Chem Soc 137 1809-16 (2015)
PMID: 25579778

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190387 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Iron hydrogenase small subunit domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 514 amino acids
Theoretical weight: 55.28 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9FYU1 (Residues: 1-497; Coverage: 100%)
Gene names: CHLRE_03g199800v5, hyd1, hydA, hydA1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SF4 2 x SF4
Ligand CL 4 x CL
Ligand ARS 2 x ARS
2 modified residues:
Ligand CSD 2 x CSD
Ligand CSO 4 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21
Unit cell:
a: 77.645Å b: 70.964Å c: 94.686Å
α: 90° β: 91.91° γ: 90°
R-values:
R R work R free
0.209 0.206 0.252
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Photobiocatalysis: Activating Redox Enzymes by Direct or Indirect Transfer of Photoinduced Electrons.
Lee et al. (2018)
7 more