4r05

X-ray diffraction
2.1Å resolution

Crystal structure of the refolded DENV3 methyltransferase

Released:

Function and Biology Details

Reactions catalysed:
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-111140 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nonstructural protein NS5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 267 amino acids
Theoretical weight: 30.07 KDa
Source organism: Dengue virus 3
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C1KBQ3 (Residues: 2491-2752; Coverage: 8%)
Sequence domains: FtsJ-like methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P1
Unit cell:
a: 44.688Å b: 48.386Å c: 68.073Å
α: 79.11° β: 78.75° γ: 69.71°
R-values:
R R work R free
0.253 0.25 0.307
Expression system: Escherichia coli BL21(DE3)