4qze

X-ray diffraction
2.25Å resolution

Mouse Tdt, F401A mutant, in complex with a DSB substrate, C-G base pair

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA nucleotidylexotransferase Chain: A
Molecule details ›
Chain: A
Length: 400 amino acids
Theoretical weight: 45.63 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P09838 (Residues: 132-510; Coverage: 74%)
Gene names: Dntt, Tdt
Sequence domains:
Structure domains:
5'-D(*AP*AP*AP*AP*AP*C)-3' Chains: D, U
Molecule details ›
Chains: D, U
Length: 6 nucleotides
Theoretical weight: 1.81 KDa
Source organism: Mus musculus
Expression system: Not provided
5'-D(*TP*TP*TP*TP*TP*GP*GP*G)-3' Chain: T
Molecule details ›
Chain: T
Length: 8 nucleotides
Theoretical weight: 2.46 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2
Unit cell:
a: 58.033Å b: 71.608Å c: 113.948Å
α: 90° β: 95.19° γ: 90°
R-values:
R R work R free
0.194 0.192 0.218
Expression systems:
  • Escherichia coli
  • Not provided