› 4qyo

X-ray diffraction
1.21Å resolution

Crystal Structure of anti-MSP2 Fv fragment (mAb6D8)in complex with MSP2 14-22

Released:
DOI: 10.2210/pdb4qyo/pdb
PDB entry 4qyo coloured by chain, front view.
PDB entry 4qyo coloured by chain, side view.
PDB entry 4qyo coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for epitope masking and strain specificity of a conserved epitope in an intrinsically disordered malaria vaccine candidate.
Morales RAV, MacRaild CA, Seow J, Krishnarjuna B, Drinkwater N, Rouet R, Anders RF, Christ D, McGowan S, Norton RS
Sci Rep 5 10103 (2015)
PMID: 25965408

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-235101 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fv fragment(mAb6D8) heavy chain Chain: A
Molecule details ›
Chain: A
Length: 114 amino acids
Theoretical weight: 12.49 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fv fragment(mAb6D8) light chain Chain: B
Molecule details ›
Chain: B
Length: 111 amino acids
Theoretical weight: 11.96 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Merozoite surface protein 2 Chain: Q
Molecule details ›
Chain: Q
Length: 11 amino acids
Theoretical weight: 1.15 KDa
Source organism: Plasmodium falciparum K1
Expression system: Not provided
UniProt:
  • Canonical: P50498 (Residues: 33-41; Coverage: 4%)
Gene names: MSA2, MSP2, PF3D7_0206800, PFB0300c

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P21
Unit cell:
a: 41.941Å b: 60.712Å c: 43.703Å
α: 90° β: 106.68° γ: 90°
R-values:
R R work R free
0.152 0.151 0.17
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Applications of (19)F-NMR in Fragment-Based Drug Discovery.
Norton et al. (2016)
9 other articles

1 mention without citation

Disordered epitopes as peptide vaccines.
MacRaild et al. (2018)