PDBe 4qyl

X-ray diffraction
1.8Å resolution

Crystal Structure of the human BRPF1 bromodomain in complex with a histone H2AK5ac peptide

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Peregrin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 117 amino acids
Theoretical weight: 13.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P55201 (Residues: 629-742; Coverage: 9%)
Gene names: BR140, BRPF1
Sequence domains: Bromodomain
Structure domains: Bromodomain-like
Histone H2A type 1 Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 12 amino acids
Theoretical weight: 1.22 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P0C0S8 (Residues: 2-13; Coverage: 9%)
Gene names: H2AFC, H2AFD, H2AFI, H2AFN, H2AFP, HIST1H2AG, HIST1H2AI, HIST1H2AK, HIST1H2AL, HIST1H2AM

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 60.908Å b: 55.581Å c: 82.135Å
α: 90° β: 93.58° γ: 90°
R-values:
R R work R free
0.206 0.204 0.253
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided