4qyi

X-ray diffraction
1.95Å resolution

1.95 Angstrom resolution crystal structure of a hypoxanthine-guanine phosphoribosyltransferase (hpt-2) from Bacillus anthracis str. 'Ames Ancestor' with HEPES molecule in the active site

Released:
Source organism: Bacillus anthracis
Entry authors: Halavaty AS, Minasov G, Dubrovska I, Winsor J, Shuvalova L, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed:
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
homo dimer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Hypoxanthine phosphoribosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 186 amino acids
Theoretical weight: 21.13 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A6L8PVA4 (Residues: 1-183; Coverage: 100%)
Gene names: GBAA_5074, hpt2
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold
Hypoxanthine phosphoribosyltransferase Chains: B, C, D, E
Molecule details ›
Chains: B, C, D, E
Length: 186 amino acids
Theoretical weight: 21.2 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A6L8PVA4 (Residues: 1-183; Coverage: 100%)
Gene names: GBAA_5074, hpt2
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: C2
Unit cell:
a: 172.779Å b: 117.009Å c: 56.883Å
α: 90° β: 90.96° γ: 90°
R-values:
R R work R free
0.223 0.221 0.252
Expression system: Escherichia coli BL21(DE3)