4qx3

X-ray diffraction
2.9Å resolution

Cry3A Toxin structure obtained by injecting Bacillus thuringiensis cells in an XFEL beam, collecting data by serial femtosecond crystallographic methods and processing data with the CrystFEL software suite

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pesticidal crystal protein Cry3Aa Chain: A
Molecule details ›
Chain: A
Length: 587 amino acids
Theoretical weight: 66.6 KDa
Source organism: Bacillus thuringiensis serovar tenebrionis
Expression system: Bacillus thuringiensis serovar israelensis
UniProt:
  • Canonical: P0A379 (Residues: 58-644; Coverage: 91%)
Gene names: bt13, cry3A, cry3Aa, cryC, cryIIIA(a), cryIIIa
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLAC LCLS BEAMLINE CXI
Spacegroup: C2221
Unit cell:
a: 117.3Å b: 135.3Å c: 105.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.177 0.194
Expression system: Bacillus thuringiensis serovar israelensis