X-ray diffraction
2.52Å resolution

Crystal structure of PTPN3 (PTPH1) in complex with a dually phosphorylated MAPK12 peptide


Function and Biology Details

Reactions catalysed:
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein phosphatase non-receptor type 3 Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 34.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P26045 (Residues: 628-909; Coverage: 31%)
Gene names: PTPH1, PTPN3
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily
Mitogen-activated protein kinase 12 Chain: B
Molecule details ›
Chain: B
Length: 9 amino acids
Theoretical weight: 1.24 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: P53778 (Residues: 182-190; Coverage: 3%)
Gene names: ERK6, MAPK12, SAPK3

Ligands and Environments

No bound ligands
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL15A1
Spacegroup: P3121
Unit cell:
a: 75.491Å b: 75.491Å c: 109.605Å
α: 90° β: 90° γ: 120°
R R work R free
0.199 0.197 0.245
Expression systems:
  • Escherichia coli
  • Not provided